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Literature summary extracted from

  • Ching, S.L.; Gidda, S.K.; Rochon, A.; van Cauwenberghe, O.R.; Shelp, B.J.; Mullen, R.T.
    Glyoxylate reductase isoform 1 is localized in the cytosol and not peroxisomes in plant cells (2012), J. Integr. Plant Biol., 54, 152-168.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.26 exclusive localization in the cytosol of transgenic Arabidopsis plants co-expressing GFP-GLYR1 and and Cherry-PTS1, a fusion protein consisting of the Cherry fluorescent protein linked to the PTS1 of the peroxisomal enzyme hydroxypyruvate reductase. Expression of N-terminal GFP-tagged or Myc-tagged GLYR1 in tobacco BY-2 cell cytosol. GFP- or Myc-tagged GLYR1 is competent, at least partially, for import into peroxisomes, since replacement of the C-terminal glutamate in GLYR1 with leucine, which yields a canonical PTS1 (i.e., a C-terminal small-basic-hydrophobic tripeptide motif), results in the modified fusion protein (GFPGLYR1-E to L and Myc-GLYR1-E to L) being dual localized to the cytosol and peroxisomes in BY-2 cells Arabidopsis thaliana
1.1.1.79 exclusive localization in the cytosol of transgenic Arabidopsis plants co-expressing GFP-GLYR1 and and Cherry-PTS1, a fusion protein consisting of the Cherry fluorescent protein linked to the PTS1 of the peroxisomal enzyme hydroxypyruvate reductase. Expression of N-terminal GFP-tagged or Myc-tagged GLYR1 in tobacco BY-2 cell cytosol. GFP- or Myc-tagged GLYR1 is competent, at least partially, for import into peroxisomes, since replacement of the C-terminal glutamate in GLYR1 with leucine, which yields a canonical PTS1 (i.e., a C-terminal small-basic-hydrophobic tripeptide motif), results in the modified fusion protein (GFPGLYR1-E to L and Myc-GLYR1-E to L) being dual localized to the cytosol and peroxisomes in BY-2 cells Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.26 cytosol GLYR1 is exclusively located in the cytosol Arabidopsis thaliana 5829
-
1.1.1.26 additional information GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress Arabidopsis thaliana
-
-
1.1.1.79 cytosol GLYR1 is exclusively located in the cytosol Arabidopsis thaliana 5829
-
1.1.1.79 additional information GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.26 glycolate + NAD+ Arabidopsis thaliana
-
 glyoxylate + NADH + H+
-
 r
1.1.1.26 glycolate + NADP+ Arabidopsis thaliana
-
 glyoxylate + NADPH + H+
-
 r
1.1.1.79 glycolate + NAD+ Arabidopsis thaliana
-
 glyoxylate + NADH + H+
-
 r
1.1.1.79 glycolate + NADP+ Arabidopsis thaliana
-
 glyoxylate + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.26 Arabidopsis thaliana Q9LSV0
-
-
1.1.1.79 Arabidopsis thaliana Q9LSV0
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.26 cell suspension culture
-
 Arabidopsis thaliana
-
1.1.1.26 leaf
-
 Arabidopsis thaliana
-
1.1.1.26 seedling
-
 Arabidopsis thaliana
-
1.1.1.79 cell suspension culture
-
 Arabidopsis thaliana
-
1.1.1.79 leaf
-
 Arabidopsis thaliana
-
1.1.1.79 seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.26 glycolate + NAD+
-
 Arabidopsis thaliana glyoxylate + NADH + H+
-
 r
1.1.1.26 glycolate + NADP+
-
 Arabidopsis thaliana glyoxylate + NADPH + H+
-
 r
1.1.1.79 glycolate + NAD+
-
 Arabidopsis thaliana glyoxylate + NADH + H+
-
 r
1.1.1.79 glycolate + NADP+
-
 Arabidopsis thaliana glyoxylate + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.26 glyoxylate reductase isoform 1
-
 Arabidopsis thaliana
1.1.1.26 GLYR1
-
 Arabidopsis thaliana
1.1.1.79 glyoxylate reductase isoform 1
-
 Arabidopsis thaliana
1.1.1.79 GLYR1
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.26 NAD+
-
 Arabidopsis thaliana
1.1.1.26 NADH
-
 Arabidopsis thaliana
1.1.1.26 NADP+
-
 Arabidopsis thaliana
1.1.1.26 NADPH
-
 Arabidopsis thaliana
1.1.1.79 NAD+
-
 Arabidopsis thaliana
1.1.1.79 NADH
-
 Arabidopsis thaliana
1.1.1.79 NADP+
-
 Arabidopsis thaliana
1.1.1.79 NADPH
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.1.1.26 additional information due to the glutamate at the -1 position, GLYR1 C-terminal tripeptide, -SRE, does not function as a type 1 peroxisomal targeting signal, PTS1. GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress Arabidopsis thaliana
1.1.1.26 physiological function GLYR1 scavenges succinic semialdehyde and glyoxylate that escape from mitochondria and peroxisomes, respectively Arabidopsis thaliana
1.1.1.79 additional information due to the glutamate at the -1 position, GLYR1 C-terminal tripeptide, -SRE, does not function as a type 1 peroxisomal targeting signal, PTS1. GLYR1 is not relocalized from the cytosol to peroxisomes in response to abiotic stress Arabidopsis thaliana
1.1.1.79 physiological function GLYR1 scavenges succinic semialdehyde and glyoxylate that escape from mitochondria and peroxisomes, respectively Arabidopsis thaliana